Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 5. BMP gene expression in the pharyngeal pouches during epibranchial development, and defects in cas mutants. Lateral views, anterior to the left. Whole- mount in situ hybridization was performed in wild types at 22 hpf (top row), 30 hpf (second row) and 40 hpf (third row), and in cas mutants at 40 hpf (bottom row), for bmp2a (A,E,I,M), bmp2b (B,F,J,N), bmp4 (C,G,K,O) and bmp5 (D,H,L,P). Arrows indicate initial expression at the ventrolateral edges of each pouch. Arrow in H denotes bmp5 expression in the dorsal pouches. 1-5, pharyngeal pouches; ot, otic capsule.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: Gene Expression, In Situ Hybridization, Expressing

Journal: Development (Cambridge, England)

Article Title: Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish.

doi: 10.1242/dev.01936

Figure Lengend Snippet: Fig. 8. BMP4-coated beads induce, and NOG-coated beads inhibit, epibranchial formation. Lateral views, anterior to the left. In situ hybridization for phox2b mRNA to label epibranchials at 48 hpf. (A) Wild-type control embryo. (B-F) Embryos that received Affi-Gel Blue beads soaked in either PBS (B), BMP4 (10 μg/ml, C; 1 μg/ml, D) or NOG protein (500 pg/ml, E; 300 pg/ml, F). Arrows in C and D indicate ectopic phox2b+ cells adjacent to the bead; arrows in E and F denote missing ganglia adjacent to the NOG-coated bead. Asterisks indicate beads. gVII, facial; gIX, glossopharyngeal; gX, vagal; ot, otic vesicle. Scale bar: 50 μm.

Article Snippet: Human recombinant BMP4, BMP5, BMP7 and Noggin proteins (R&D Systems) were used for bead implantation experiments.

Techniques: In Situ Hybridization, Control

Journal: eLife

Article Title: Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos

doi: 10.7554/eLife.59157

Figure Lengend Snippet: ( A ) Schematic summary of caudal PNS specification and molecular regulators in Ciona intestinalis (adapted from ; ; ; ; , more details can be found in ). ( B–L ) Expression of the P. mammillata orthologs of the C. intestinalis caudal PNS genes. In situ hybridization of a late neurula for Msx ( B ), at early tailbud stages for Ascl.b ( C ), Klf1/2/4 (D), Nkxtun3 (E), Tox ( F ), Bhlhtun1 ( G ), Dlx.c ( H ) and Dlk ( I ); and at mid/late tailbud stages for Atonal ( J ), Pou4F ( K ), and Celf3.a ( L ). ( M–Q ) Expression of Phmamm-Klf1/2/4 at early tailbud stages in control embryos ( M ), following electroporation of pFog >Ciinte.Admp ( N ) or pFog >Ciinte.Noggin ( P ); or following treatment with Bmp4 protein ( O ) or Dorsomorphin ( Q ). ( R–U ) Expression of Phmamm.Pou4F at late mid/late tailbud stages in control embryos ( R ), following treatment with Bmp4 protein ( S ), DAPT ( T ) or a combination of Bmp4 and DAPT ( U ). Embryos are shown in lateral view with dorsal to the top and anterior to the left. Scale bars: 50 μm.

Article Snippet: P. mammillata embryos were treated with 150 ng/ml recombinant zebrafish Bmp4 protein (1128-BM, R&D Systems Inc, stock solution in HCl 4 mM + BSA 0,1%) complemented with 0.1% BSA from the eight-cell stage, with 20 μM of the Bmp receptor inhibitor Dorsomorphin (S7306, Euromedex, stock solution in water) from early gastrula stages or with 25 μM of the γ-secretase inhibitor DAPT (D5942, Sigma-Aldrich, stock solution in DMSO) from early neurula stages.

Techniques: Expressing, In Situ Hybridization, Control, Electroporation